5M甜菜堿溶液-PCR級(jí)
5MBetaine Solution-PCR級(jí)
● 產(chǎn)品貨號(hào)及規(guī)格：
|
貨號(hào)
|
名稱
|
規(guī)格
|
貯存
|
|
RTE3103-02
|
5M 甜菜堿溶液
|
5×1 ml
|
-20℃
|
● 產(chǎn)品簡介：
Betaine, DMSO and formamide helps in reducing the secondary
structure of GC rich templates and assists amplification of these templates.
Betaine is used at3.5Mto0.1M, DMSO should be used between 2-8%,
however 10% DMSO can reduce Taq polymerase activity by up to 50%. Formamide is
generally used at 1-5%. It has been our experience that the use of DMSO in
combination with betaine is superior to using formamide.
● 貯存：-20℃貯存。有效期一年。
● 使用說明：
1．-20℃保存的甜菜堿溶液常溫融化，混勻。
2. 根據(jù)根據(jù)PCR反應(yīng)體系，加入適量體積的5M甜菜堿溶液。
|
|
20 μl 體系
|
反應(yīng)體系終濃度
|
|
10×PCR Buffer
|
2 μl
|
1×
|
|
5M Betaine*
|
4 μl
|
1 M
|
|
模板
|
0.05-0.5μg
|
as you wish
|
|
上游引物 10μM
|
0.5 μl
|
0.2 μM
|
|
下游引物 10μM
|
0.5 μl
|
0.2 μM
|
|
dNTP10mM
|
0.5 μl
|
200 μM
|
|
Taq
|
0.5-1 μl
|
2.5-5 U
|
|
滅菌水
|
up to 20 μl
|
|
注*：不同模板，不同片段的擴(kuò)增可能需要不同濃度的甜菜堿，可以適當(dāng)調(diào)節(jié)加入體積，找到適合自己反應(yīng)的甜菜堿濃度。
● References：
1 Rees, William A., et al., Betaine can eliminate the
base pair composition dependence of DNA melting. Biochemistry, 32, 137-144 (1993)